The change to a cooler, dry, seasonal climate had considerable impacts on Pliocene vegetation, reducing tropical species worldwide. Deciduous forests proliferated, coniferous forests and tundra covered much of the north, and grasslands spread on all continents (except Antarctica). Tropical forests were limited to a tight band around the equator, and in addition to dry savannahs, deserts appeared in Asia and Africa.
Both marine and continental faunas were essentially modern, although continental faunas were a bit more primitive than today. The first recognizable hominins, the australopithecines, appeared in the Pliocene.
The land mass collisions meant great migration and mixing of previously isolated species, such as in the Great American Interchange. Herbivores got bigger, as did specialized predators.