Because Atlan activated the False Mutation of his Canzer Polyglot mutation, the Queen was able to understand his words even though he was using human language.
She was speechless with his audacity. A mere human treated her as nothing but an oversized lizard. The thought was so absurd that the Queen wasn't immediately offended but felt that it was a little bit amusing.
'I wonder where you get your confidence, human. Careful. If I wasn't so magnanimous, I would have crushed your skull along with the humans back there.'
Atlan, instead of answering, continued to put his attention towards Ninth, who was bleeding out of every orifice. He had to rip off part of her battle suit just to make a make-shift bandage to at least patch her up a bit.
"Porter…what are you…doing—COUGH!'
Ninth wanted Atlan to escape with Tulip before the Queen had the chance to get her hands on him. She didn't want him to end up like him.