After using the serum on Li, she spent some time preparing several for different other animals as she worked with Dr Indigo and Tristan on the SIQ serums for them.
Each of the crew members were having their own difficulties and challenges out in the world as Alyssia received more updates about those being injured during battle as the difficulty increased after many had escaped from many of the floors of Impel Down while some of them were previously notorious pirates from a long time ago.
Some were still able to display their previous strengths while some had been reduced in power from many years of imprisonment and ageing.
After being suppressed for too long, upon being free they had released their desires upon anyone that crossed them whether it be the ordinary citizens, businesses, nobles, anyone with some form of authority or the Marines and other influences.
The White Storm had done well to hold them off so far but that did not mean that they could hold on forever as there would eventually be more serious injuries to them or even death for many.
The ships that had one of the main crew members on board or sea creatures to help protect the ships were better off than others as they were able to gain much better protection.
Even the underworld of Rica Island was experiencing a slight reshuffling of power as the lawless king used many tactics to make his least loyal people or traitors among his alliance face off against the new people that had arrived which allowed him to deal with the new threat and stabilise his forces a little.
Around the world, many things had continued to happen like Garp's return to the East Blue, Akainu and Kuzan's battle which resulted in Kuzan defecting from the Marines, Blackbeard leading his crew to steal Devil Fruits from people in the New World while he was searching for a suitable replacement for the Quake Devil Fruit that he had failed to acquire.
Punk Hazard which was the site of their battle was transformed completely into a land split up by the two opposing powers of Ice and Magma while Caesar Clown would soon occupy it for his own purposes.
Another problem happened within the Marines which was related to the training instructor Zephyr who had many of his trainees wiped out and suffered more grievances as he had to swallow his anger which would soon lead to him leaving to form the Neo Marines.
As everything was going on in Rica Island or around the Grand Line, those within the Sunken Island remained unaffected by most of it but upon reading the newspaper, Alyssia frowned as her eyes fell upon something interesting. "Hmm.. so that time will soon be approaching."
She threw the newspaper aside and then walked towards a shelf where there was a row of metal cans which she picked up one of them with a black colouring.
She started rolling it back and forth for a while before placing it in front of herself. "Soon I will be able to use this."
She then took out a Sea Snail and spoke. "Keep a lookout for the actions and movements of the Buggy Pirates then keep me updated if they show any changes. If possible try to find a way to get to some mercenary groups that are looking for work before they approach his group too."
Buggy had started using his new power as a Warlord to work on forming the Buggy Mercenaries but it was still in its early stages so it could not possibly be formed so soon to be able to compete with the White Storm Mercenaries even with the many loyal escaped prisoners on Buggy's side.
With Buggy's fame, he would attract many strong combatants within the two year time skip but Alyssia did not wish for those with high combat power to go to him especially the group of Giants so she had them monitored from within.
With the increasing number of conflicts around the Grand Line, there would soon be many emerging powers in Paradise or the New World while Luffy was learning Haki with Rayleigh.
He as gradually adapting to Armament Haki after recovering from his wounds but he could not yet fully grasp it even after enduring hellish training under Rayleigh and fighting strong beasts day and night.
Just as he was gradually getting used to it, the outside world was disturbed as Byrnndi World reunited with his former crew the World Pirates as they started to reemerge in the world which resulted in the calling of the Warlords to prevent further destruction.
To counter the World Governments move against them, they targeted the Pirate Empress Boa Hancock so that they could use her as a hostage which resulted in them arriving on the island that Luffy was training on while Boa Hancock's sisters were there to leave Luffy some things to help him survive and live on the island.
After recovering from his defeat from Byrnndi World, Luffy and Hancock left to confront their crew while Buggy had soon arrived followed by Mihawk.
After several defeats, Luffy was able to briefly use Armament Haki during a moment of becoming enraged towards Byrnndi World and was able to knock him down.
During their final confrontation, Luffy was able to produce a Red Hawk attack to defeat him and escape.
However what many did not notice was that a certain individual had snuck into the battlefield and started purchasing the surrounding skills from everyone whether it be Gairam's use of hammer techniques, shipbuilding or the Fishmen Karate from Sebastian along with many other fighting techniques from those around her like those from Byrnndi World.
However her main purpose was not to be caught so she did not stick around for long as she had remained invisible because of the suit on her body made with Germa technology.
Before the event had reached the climax and just after Boa Hancock had defeated the ship doctor Nightin by turning her to stone, Alyssia entered the room after Hancock had left and grabbed ahold of the stone body which was still living even after being petrified.
She lifted it onto her shoulders and then quickly covered it with her cape to provide some invisibility before escaping the ship and flying away with her before the Marines destroyed the ship and killed all those from the World Pirates.
Alyssia looked towards the destruction brought on by Mihawk's sword and Akainu's magma punches and then fled back towards Rica island with her newly acquired doctor which had a large variety of herbal knowledge including her own rejuvenating mix and many other types of mixtures for different purposes.
Not only were her medical skills useful but her Kanpo Kenpo style of combat which uses medicinal herbs and poison to attack her opponents and enhance her own strength.
Not only was she skilled enough in her old age to take down many Marines but she was able to fight on par with Boa Hancock so Alyssia had to bring her back.
Since Boa Hancock had turned Nightin into stone, she would remain alive in a frozen state until released so she was just a frozen set of skills to Alyssia to use until the stone body can no longer be preserved so she was careful not to damage it as she landed back in Rica Island.
Dr Indigo had previously provided Tristan with a new way to improve her fighting style and combat effectiveness even further but upon acquiring the stone statue of Nightin, Alyssia saw even more possibilities for Tristan's style of combat.
Tristan was not a pure martial artist or combatant but she was a capable fighter due to her Mink race which allowed her to defeat many opponents with ease and grow in strength quickly but her main role was always that of a doctor.
If she could apply her medicinal skills from everything she had learned into a similar way of using the style of Nightin with her own martial arts then she would be able to grow even stronger and with Alyssia's persistent training of the non-Haki users of a young age, those among the Minks were slowly beginning to awaken and grasp it one after another so each of them would become the main force of the crew along with Sunila, Monera, Jester and Liushi while Medea was rising up with her newly gained abilities from her Rumble Logia Devil Fruit and some had more talent towards Observation like Aizah, Nica and Ashwin, most others preferred Armament or trained in both.
With her newly gained knowledge, Alyssia started to continue her work on different types of serums and medicinal solutions with Tristan and Dr Indigo with Nightin by their side.