Graduation Physics and Chemistry
Volume 47, Issue 3, March 1996, Pages 501-505
Effects of X- and UV-irradiation on proteins
Author links open overlay panelH.Durchschlag1P.Zipper2
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Abstract
The radiation damage of several proteins has been investigated in aqueou solution, after preceding X- or UV-irradiation. In order to differentiate between different radiation effects, a series of physico-chemical and biochemical methods as well as novel evaluation and normalization procedures have been applied. Numerous changes of several molecular parameters characterizing functional ability and primary, secondary, tertiary and quaternary structure of proteins could be found. Modifications of radiation effects were achieved by a variety of measures and additives. The results are of interest for many fields of research using X-rays or UV light.Radiation Physics and Chemistry
Volume 47, Issue 3, March 1996, Pages 501-505
Effects of X- and UV-irradiation on proteins
Author links open overlay panelH.Durchschlag1P.Zipper2
Show more
Outline
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https://doi.org/10.1016/0969-806X(95)00138-NGet rights and content
Abstract
The radiation damage of several proteins has been investigated in aqueou solution, after preceding X- or UV-irradiation. In order to differentiate between different radiation effects, a series of physico-chemical and biochemical methods as well as novel evaluation and normalization procedures have been applied. Numerous changes of several molecular parameters characterizing functional ability and primary, secondary, tertiary and quaternary structure of proteins could be found. Modifications of radiation effects were achieved by a variety of measures and additives. The results are of interest for many fields of research using X-rays or UV light.Anew type high spatial resolution radiation detector based on UV scintillators + UV imaginggas photomultiplier is presented. The prototype UV detector consists of a 10cm×10cm μPIC,2GEMs,aUV transparent windowandaCsI photocathode deposited on the window or the surface of the upper GEM. The effective photo-sensitive area is ø34mm andthe readouts are 400um-pitch strips.Anewlydeveloped LuLiF4(Nd) crystal which emits 183nm photonsis directly coupled to the detector as the window. The detector was tested in pulse mode operation with 5.5MeV α particles from 241Am and 122keV γ rays from 57Co. The window images were clearly obtained and the position resolution of o(mm) is confirmed.Ultraviolet light (UV) forms part of the electromagnetic spectrum, and it can be harnessed to inactivate microorganisms associated with foods and food-processing operations. The most effective wavelengths for direct inactivation of microorganisms lie in the so-called UV-C region of the electromagnetic spectrum. A number of UV sources are in current use, but low-pressure mercury sources are relatively efficient emitters of germicidal UV and these sources traditionally have been used to disinfect liquids, air, and the surfaces of solids, including foods. UV has also been used in conjunction with other treatments, most notably peroxidation, to bring about synergistic disinfection. Systems can be put in place to enable a wide variety of foods to be treated with UV in such a way as to avoid unnecessary overexposure which might result in loss of quality.