After several days of observation, Kuroto was able to confirm that the two pairs of Mangekyou Sharingan in the eye sockets of Ryota have begun a preliminary fusion, however, the fusion speed is very slow.
Standing in front of the test bench, Kuroto help his chin with his right hand and his left hand over his chest **thinking posture** and muttered to himself, "At this speed, both the pairs will take at least three months to fully fuse into Eternal."
Ryota, the host carrying out the fusion, is in a state of Coma, so the fusion of two pairs is not being guided by his will like a voluntary process, rather it is taking place as per his subconscious.
Because of this, the fusion of both the pairs into Eternal Mangekyou Sharingan has slowed down.
And Kuroto does not seem to have any effective solution to solve this problem and has to calmly wait.
As for whether the two pairs will truly fuse successfully or whichever techniques will the Eternal Mangekyou Sharingan awaken, Kuroto no longer has any say in this, it all depends on the end result.
So, putting away the matter of the Eternal Mangekyou Sharingan; for now, Kuroto started to begin the plan for the cultivation of the Fire Nature Clone.
The difficulty of Cultivating the Fire Nature Clone is undoubtedly much higher than the previous Water nature Clone.
This is not only because Fire Nature is extremely violent and burning and therefore is not as stable as the Water Nature but also because the subject whose genes Kuroto has chosen for the fire Nature Clone is a member of the Uchiha Clan.
Although the Uchiha Clan is born with a natural affinity for Fire Release, it is still considered the secondary chakra nature, while Yin Nature is a more common Chakra Natures that the members of the Uchiha Clan have, and Kuroto also has extremely dense and potent Yin Chakra, which symbolizes spiritual energy and is necessary for Dojutsu-type Kekkei Genkai.
As such during the whole cloning process, Kuroto not only has to filter out his own Yin Chakra but Shisui's Yin Chakra as well to make Fire Chakra nature the main nature of the resulting clone, otherwise the clone would become a waste of effort and resources.
Kuroto already has some experience in filtering out the Yin Chakra because he filtered out his own Yin Chakra while creating Water Nature Clone, as such he understands that the process of Chakra Nature filtration causes some degree of damage to the cells.
At that time, only Kuroto's cells were required to be filtered while Yuki Haku's cells were used as is, but this time both his and Shisui's cells have to be filtered.
In other words, the damage to cells will be higher, which will reduce the probability of successful fusion of the two cell types.
Buzzing…
At this time the alarm from the Large Centrifuge filter sounded indicating that the cell samples he placed in the centrifuge filter for the Yin Chakra filtration of both his cells and Shisui's cells have been completed.
Initially, Kuroto prepared 500 samples from his cells. Since Kuroto already has a certain level of experience filtering out Yin Chakra from his cells, therefore, he had a better understanding of which potencies to use to not damage the cells too much. As such more samples survived the filtration process than the last time, the number stretched to 89!
That is correct, the total number of samples that survived the filtration process is 89.
As for the rest?
They are either too damaged or completely lost their cell activity.
Similarly, Kuroto prepared another 1000 samples from Shisui's cells. He prepared twice the number because this was the first time, he carried out Chakra filtration for Uchiha's cells, as such he had to work his way through various potencies and find which one suited the cells samples perfectly, this again was manual work and took a lot of days.
Finally, the number of samples that survived is 73.
These 89 and 73 samples were deemed suitable to be processed and used for the next step which is the cell fusion process while the rest were discarded and disposed of.
Without any further ado, Kuroto started the cell fusion process
Similar to last time, the ratio of the number of cells he used for fusion was 99:1.
In simple words, for each cell of Shisui, 99 of his cells were used.
This means, that the stem cells that will be used to create the cell bud for the cultivation of Fire Nature Clone will have 99% of Kuroto's cells and 1% of Shisui's cells.
For those wondering how is that even possible, as the number of cell samples does not match this ratio?
The answer is very simple.
Before we talked about the number of cell samples, not the number of cells, each sample contains more than one cell as such maintain the ratio is very easy.
The final number of samples that went through the fusion process is 73.
After injecting Shisui's cells into the 73 samples one-by-one, Kuroto quietly waited for the fusion process to begin.
Soon, failed result started to appear, which means that the cell fusion did not occur, and resulted in a rejection, there could be multiple causes for this, which includes, insufficient cell activity, unmatchable DNA, death of the fused cells midway through the fusion process and so on.
In less than an hour, the fusion reaction of 59 samples resulted in a failure.
In the next few minutes, the leftover 14 samples also started to lose cell activity, by the time the entire fusion process was over for all the samples, only one sample survived the fusion process, while the rest all were dead.
Kuroto who was observing the entire fusion reaction had a serious look on his face.
During the cloning of Suijin Clone, the number of samples that went through the fusion process was only 6, and even out of those 6, 3 samples successfully survived the fusion process, showing a success rate of 50%.
But this time, 73 samples went through the fusion process, and still, only 1 sample survived the fusion process, the success rate of the experiment is not even 1.37%.
"Does the DNA combination of Hyuga and Uchiha has such a high rejection rate?"
Although Kuroto expected that cultivating Fire Nature Clone would not be easy, and would require repeated trial and error even during the cloning process, he did not expect that the success rate of the fusion process would drop down to such a low percentage.
Sighing slightly, Kuroto collected the only sample that survived the fusion process, named it Sample-1A, placed it on top of the stage clip of the high precision microscope, and observed it through the Ocular Lens.
While observing the fused group of cells, Kuroto's face was full of disappointment.
This is because the only sample that successfully survived the fusion process is also very severely damaged, and the cell activity is also very low, as such it does not fit the minimum standard necessary for creating the cloning bud and was therefore classified as unsuitable.
But Kuroto thought a little and after a while of hesitation, Kuroto decided to not discard it but put it inside the incubator to start the budding.
In the following weeks, Kuroto continued this experimentation, Chakra filtration, fusion, and so on, and the results were far worse than he expected, no matter how many samples were used or how many samples went through the fusion process, none of them were deemed successful, each and every reaction for the fusion process resulted in failure!
This became a reason for Kuroto's distress, even after spending so much time, money, effort, and energy, the result he obtained were repetitive failures, finally, he couldn't help but wonder, "Is it possible my and Shisui's genes are noncompatible that the fusion process is resulting in all but failures?"
This doubt is not unreasonable, the success rate of the previous fusion experiment was as high as 50%, but the overall success rate this time is not even 0.1%, and gene incompatibility is the only reason that Kuroto can only think of for such a result.
And strangely enough, while Kuroto continued his experimentation process, the cloning bud that was made out of the Sample-1A (which Kuroto deemed to be unsuitable but did not discard) and was placed in the Large nutrition tank to undergo the cultivation process was lucky enough to develop.
However, Kuroto would always have a frown whenever he would observe this developing Clone.
Because Kuroto can't understand why this unsuitable sample underwent some kind of change as a result of which, its gender changed into that of a female as it developed from the stem cells to its current state, completely deviating from Kuroto's plan.
Naturally, Kuroto can't accept using this female clone, so he had to continue the cloning experimentations, with different cell samples including, Itachi, Shinichi, etc.
Now, Kuroto no longer feels that he has so many choices to choose from, because, the rejection between Uchiha and Hyuga genes is extremely very high!
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Read up to Chapter - 503 on P.a.t.r.e.o.n. Page: p-atreon.com/Milta_translations