After completing the Animax Soul Rune made with Xenia's True Soul, Zatiel rested for a couple of hours before beginning to work in the next one.
Zitu's True Soul was not in a comatose state like that of Xenia, but it was still pretty injured, so after using his Rebirth Eye, Zatiel managed to weaken it enough so it could not put any resistance.
The process went smoothly, and before the day ended, a new pentagram rune appeared in front of Zatiel. However, unlike the previous one, this had a ghargatula sealed in the center.
The Neo-Demon stored the rune in a unique orb, just like he did with the last one.